Proteomic analysis of the chondrocyte cell surface

Zhang, Xiaofei (2011) Proteomic analysis of the chondrocyte cell surface. MRes thesis, University of Nottingham.

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Abstract

Osteoarthritis, which is characterized by the progressive degradation of articular cartilage, is one of the major challenges for medical science because of its prevalence and lack of effective therapy. Currently, many scientists are interested in the biology of chondrocytes in health and disease because they play key roles in cartilage degradation. Membrane proteins are responsible for many important biological processes but they are poorly understood. Thus, relevant analysis of membrane proteins is still deficient. Thus, this project focuses on how to extract membrane proteins from chondrocytes and identifying these membrane proteins with the help of proteomics techniques.

The main aims of this project were to develop a detergent-based separation method (Triton X-114) to extract membrane proteins from equine articular chondrocytes and use nanoLC-MS/MS with two proteomics methodologies (shotgun and gel-based methods) to identify membrane proteins and evaluate the efficacy of the extraction method.

As a result, approximately 50% of the proteins identified in the hydrophobic fraction were membrane proteins, which support the efficacy of the Triton X-114 extraction method. In total, 52 membrane proteins were identified in this project, some of them play important roles in chondrocyte cell biology and even in osteoarthritis, such as integrin beta-1, CD44 and CD151.

Item Type:Thesis (MRes)
Supervisors:Mobasheri, Ali
Liddell, Susan
Faculties/Schools:UK Campuses > Faculty of Science > School of Biosciences
ID Code:2329
Deposited By:Mr Xiaofei Zhang
Deposited On:17 Oct 2012 13:28
Last Modified:17 Oct 2012 13:28

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