Investigating the genetic program of germ cell specification in pig
Sun, Xiaoni (2010) Investigating the genetic program of germ cell specification in pig. MRes thesis, University of Nottingham.
At present, as the specification of germ cell in mouse well established, mechanisms of genetic and epigenetic regulation during germ cell development become important. Because whether they are identical in other mammals was unclear, detecting the expression of genes which are identified in mouse embryo in pig seems necessary. This project aims to investigate the expression of porcine PGC markers, trace PGC movement during porcine embryo development and gain understanding of the ontogeny of germ cells in pig. With the gene profiles of porcine, novel potential PGC markers can be identified, e.g., if we get a new antibody with no understanding of expression in pig germ cells, CT-1 (membrane protein) for instance, we can utilize oct-4 (nucleus protein) to identify CT-1. Specially, the approaches of porcine embryonic study can be spreaded to other mammalian species including human, which will facilitate us to understand human germ cell development.
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