The analysis of cell fate post-ejection through parenteral devices and the development of systems that aid the transportation of cell therapy products
Agashi, Kapil (2010) The analysis of cell fate post-ejection through parenteral devices and the development of systems that aid the transportation of cell therapy products. PhD thesis, University of Nottingham.
Herein, two translational issues related to the development of cell therapy discoveries into therapeutic products, are addressed. Firstly, analysis of whether the manipulations required to transfer cells from cell culture conditions to a target tissue affect cellular characteristics was performed. It was shown that processing primary murine mesenchymal stem cells (MSCs) into a concentrated cell suspension, drawing them up into a syringe and immediately ejecting them, caused a significant viability decrease. Leaving the cells within the syringe chamber at room temperature for prolonged time periods caused to a further decrease in viability. However, cells that were viable post-ejection were found to be functional with regard to their ability to attach and proliferate. Reducing the ejection rate or using the antioxidant n-acetyl cysteine did not significantly improve viability, although using a wider bore 22g needle did improve viability.
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